Alternative Name: | HSP86, Heat shock protein 90α |
Clone: | 9D2 |
Host: | Rat |
Isotype: | IgG2a |
Immunogen: | Purified Hsp90 isolated from human therapeutic orchiectomy specimens. |
UniProt ID: | P07900 |
Species reactivity: | Human Chicken |
Applications: | WB |
Recommended Dilutions/Conditions: | Western Blot (1:2,000-1:50,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
Application Notes: | Detects a band of ~90kDa by Western blot. |
Purity Detail: | Protein G affinity purified. |
Formulation: | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide diluted in proprietary stabilizer solution. |
Handling: | Avoid freeze/thaw cycles. |
Shipping: | Blue Ice Not Frozen |
Long Term Storage: | -20°C |
Scientific Background: | The 90 kDa molecular chaperone family includes 90 kDa heat shock protein Hsp90 and 94 kDa glucose-regulated protein grp94, both major molecular chaperones of the cytosol and the endoplasmic reticulum. Mammalian cells contain isoforms Hsp90 alpha and Hsp90 beta, encoded by separate genes. The amino acid sequences of human and yeast Hsp90 alpha are 85% and 90% homologous to those of Hsp90 beta, respectively. All known members of the Hsp90 protein family are highly conserved, especially in the N -terminal and C-terminal regions containing independent chaperone sites with different substrate specificity. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 functions as part of the cell |