Alternative Name: | Heat shock protein 90 |
Clone: | 3G3 |
Host: | Mouse |
Isotype: | IgM |
Immunogen: | Purified mouse HSP90 (heat shock protein 90). |
UniProt ID: | P07901 (HSP90α), P11499 (HSP90β) |
Species reactivity: | Human, Mouse, Rat Chicken, Rainbow trout |
Applications: | IF, IHC, IP, WB |
Recommended Dilutions/Conditions: | Immunofluorescence (1:200) Immunohistochemistry (1:200) Western Blot (1:500) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
Purity Detail: | Ammonium sulfate/ascites suspension. |
Formulation: | Liquid. Contains no preservatives. |
Use/Stability: | Before use, centrifuge suspension (4’000 x g for 15 min.) and discard supernatant. Resuspend pellet in 200µl PBS, pH 7.2. If desired, add 0.05% sodium azide. Stable for 6 months after PBS resuspension.Removal of residual ammonium sulfate is not usually necessary for IP procedure; for other uses it may be removed by dialysis or equivalent method. |
Handling: | Avoid freeze/thaw cycles. |
Shipping: | Shipped on Blue Ice |
Short Term Storage: | +4°C |
Long Term Storage: | -20°C |